Unfortunately not, I would throw it away after 24 hrs thawed. Unlike meat prepared for human consumption (which can stay refrigerated for like a week or so), whole-body prey items spoil much faster due to the bacteria in their internal organs.
Think of it as serving a meal at the perfect temperature, and master these basics, and youll discover feeding tricks thatll make mealtime smoother. Never use microwaves or leave mice at room temperature too long, as bacteria love that sweet spot.
Once the rodent is completely thawed, remove it from the bag by grasping it by the tail with feeding tongs and present it to the snake. A little wiggling of the rodent may be needed to simulate life and encourage a feeding response.

While live feeding was once common, the practice of feeding frozen-thawed rodents has become the gold standard, prioritizing both the snakes safety and the ethical treatment of feeder animals.
As for timing, I usually feed in the mornings, simply because it fits my schedule. But if you're dealing with a picky feeder, try offering food right after the lights go out or in a dimly lit roomespecially for nocturnal or crepuscular species.

Feeding a rat that has been thawed for too long can lead to food poisoning, bacterial infections (like Salmonella), and other digestive issues. In severe cases, it can be fatal, especially in young or immunocompromised animals.